Few studies have systematically addressed evolutionary changes in olfactory neuron assemblies, either by genetic drift or as an

adaptation to specific odor environments. We have studied the sense of olfaction in 2 congeneric scarab beetles, Pachnoda

interrupta Olivier and Pachnoda marginata Drury (Coleoptera: Scarabaeidae: Cetoniinae), which are both opportunistic

polyphages, feeding mainly on fruit and flowers. The 2 species occur in dissimilar habitats: P. interrupta is found in dry

savannah, and P. marginata in tropical parts of equatorial Africa. To study how these species may have adapted their sense of

olfaction to their odor environments, we utilized single-unit electrophysiology on olfactory sensilla with a wide selection of

food-related compounds. Despite the differences in habitat, we found that the species shared most of the physiological types

of olfactory receptor neurons (ORNs) encountered, although their proportions frequently varied between the species. The high

degree of conservation in olfaction between the species implies that a similar sensory strategy is efficient for food search in

both habitats. However, shifts in proportions of receptor neuron classes, and slight shifts in response profiles and/or presence

of some ORN classes unique to either species, may reflect adaptation to a different set of hosts.

Key words: comparative study, electrophysiology, olfaction, polyphagous herbivore, single sensillum recordings

Few studies have addressed how olfactory systems may be adapted to different odour environments. I have performed the largest study to date, comparing olfactory receptor neurons in the two congeneric species of scarab beetle, P. marginata and P. interrupta. Both species are fruit- and flower-eaters but geographically separated (equatorial versus sub-Saharan Africa, resp.). They have similar lamellate antennae covered mostly with numerous olfactory sensilla placodea (plate sensilla) and a lesser number of other types, mainly sensilla coeloconica (grooved peg), and smooth peg sensilla of unknown function. By means of single sensillum recordings with tungsten microelectrodes I screened a great number of olfactory sensilla with a large array of odorants. I compared 456 sensilla placodea, containing 212 responding cells, over the whole antennae in both species. The olfactory systems of these two species displayed an amazing degree of conservation, with 20 identified olfactory neuron classes, all except two of which were found in both species with no detectable difference in response profiles. The exceptions were two olfactory receptor neuron classes: methyl benzoate and gamma-nonalactone, which were only found in Pachnoda marginata. In general, the two species showed an almost total overlap in their receptor neuron assemblies. One aim of the study was also to test whether the arrangement of neurons within sensilla was conserved between the species, but this could not be tested as very few combinations of characterized neurons were encountered during the study.

Key words: olfaction, electrophysiology, scarab beetles, Pachnoda marginata, Pachnoda interrupta, single sensillum recording, olfactory receptor neurons

The sorghum chafer, Pachnoda interrupta Olivier

(Coleoptera: Scarabaeidae: Cetoniinae), is a key pest on

sorghum, Sorghum bicolor (L.) Moench (Poaceae), in

Ethiopia. At present there is a lack of efficient control

methods. Trapping shows promise for reduction of the pest

population, but would benefit from the development of

attractive lures. To find attractants that could be used for

control of P. interrupta, either by mass trapping or by

monitoring as part of integrated pest management, we

screened headspace collections of sorghum and the highly

attractive weed Abutilon figarianum Webb (Malvaceae) for

antennal activity using gas chromatograph-coupled electroantennographic

detection (GC-EAD). Compounds active in

GC-EAD were identified by combined gas chromatography

and mass spectrometry (GC-MS).

The present study was performed to evaluate the anti-schistosomal activity of the medicinal plant ginger Zingiber officinale. Mice were individually infected with 100 Schistosoma mansoni cercariae. Four weeks post-infection, mice were orally treated with 1200 mg/kg of ginger for ten consecutive days. After the last dose all animals were sacrificed to evaluate the efficacy of ginger in treatment of the infection. The results obtained showed moderate reduction of 16.5% in the worm burden compared with control infected animals. The liver egg count showed a marked reduction of 53.8%. Ginger treatment showed a significant reduction in the size of liver granuloma where a percentage reduction of 66.35 was observed. Ginger treatment was slightly reflected on the liver function at such rate of infection, where an improvement in serum arginase activity was recorded, while no appreciable improvement in hepatic ALT and AST activities, albumin and creatinine contents. In conclusion Z. officinale displayed some degree of anti-schistosomal activity through reducing of the S. mansoni eggs output and the liver granuloma size.

Abstract

Reproductive tracts were collected from 870 ewes slaughtered at an abattoir in County Dublin over a 9-mo period from September 1993 to June 1994. Ovarian activity was evident in 59.3% of the tracts examined. The mean ovulation rate was 1.26 ± 0.045 (mean ± SEM). The spatial relationship between corpora lutea (CL) and dominant follicles was established. Ten percent of the ewes examined were pregnant. Embryonic transuterine migration was observed in 12% of the single ovulating ewes and in all ewes pregnant with twins which had double ovulations on one ovary. Based on the observation of tracts with contemporaneous double ovulations and a single fetus, the prevalence of embryonic loss was 17.2%. Overall, 9.4% of the tracts showed evidence of pathological change, and 5.5% of the tracts had abnormalities likely to impair fertility.