Since the Alhamada area is known for its rangelands and most of its inhabitants raise animals, especially sheep and goats, this study was conducted by collecting samples randomly from six herds of sheep distributed randomly from the specific areas of Qurayat and Ghadames This study was aimed to determine the prevalance of Eimeria, which causes coccidian in sheep's and study the effects of many factors such as age, sex, and the breeding system, it is the first study conducted in the Hamada area on this parasite, using microscopic examination, 54 samples of sheep and goat feces were examined, of different ages and of both sexes in the month of May 2022. The result showed that the infection rate was 28% in sheep and 43% in goats. It was noted that there were no significant differences for age in the infection rate, as the highest infection rate was in the age group (>2) years (39%), while the lowest infection rate was in the age group of (1-2) years (7%), it was also shown that there is no significant difference for gender on the infection rate, as the infection rate in males was (22%) and females (48%). The results showed a slight difference in the infection rate between the open and closed breeding systems in the Hamada region, with the closed system averaging 25% compared to 20% in the open system. Clinical signs also appeared on some of the infected animals, including diarrhea, general weakness, and some of them showed plucking of wool/hair.

The study aimed to present the first record of insect families in Farwa Island. Farwa Island is the largest and most important island in Libya; located in Northern west part of Libya in the Mediterranean, its insect fauna is poorly studied. Short time intensive collection of insect samples was conducted in four areas (mouth of the valley, bay of containers, center of the Island, and Ras-Attalgha) in February, March and April 2021. Different methods were used for insect collection, sorting, preservation and later identification to the lowest taxonomic level and verification. 9 orders, 31 families, 40 genera and 58 insect species were collected in the study; the center of the Island had the highest numerical density and diversity of insect fauna due to its diverse vegetation compared to other areas. Diverse suitable habitats and absent insect control measures provided suitable environmental conditions for insects breeding, feeding and hiding from enemies. This study constitutes the first comprehensive survey of insect fauna on Farwa Island and adds significant value to the ongoing assessment of insect diversity in Libya. A long-term study has to be conducted to investigate detailed information about the abundance and diversity of insects and other arthropods on Farwa Island.

The genus Segestria Latreille, 1804 and the species Segestria florentina (Rossi, 1790) are recorded in Libya for the first time, based on a single adult female specimen

Seven male specimens (one immature and six adults) of a rare species of Loureedia jerbae (El-Hennawy, 2005), are recorded for the first time in Libya, representing the first record of this species outside of Tunisia. Illustrations and a description are provided. Only the most recent adult of the specimens captured in October/November of 2019 to 2022 was examined, identified and described. Although Libya has one of the least recorded spider species in the Mediterranean region, it is promising to reveal more undiscovered and undocumented species through extensive research in this large country.

Keywords: Araneae, North Africa, Joker Spiders, Libya, Rare Species.

Abstract

Seven male specimens (one immature and six adults) of a rare species of Loureedia jerbae (El-Hennawy, 2005), are recorded for the first time in Libya, representing the first record of this species outside of Tunisia. Illustrations and a description are provided. Only the most recent adult of the specimens captured in October/November of 2019 to 2022 was examined, identified and described. Although Libya has one of the least recorded spider species in the Mediterranean region, it is promising to reveal more undiscovered and undocumented species through extensive research in this large country

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Using macroinvertebrate-based biotic indices and diversity indices to assess water quality: A case study on the Karasu Stream (Kastamonu, Türkiye)

İbrahim Küçükbasmacı, Abdulhamed M Omar Etriieki

Biomonitoring is an approach that uses indicators or sentinel species to assess the health or pollution of an environment, combining diversity based on specific taxonomic groups with the taxa’s indicator of pollution into a single index or score. Benthic macroinvertebrates are among the most preferred and valuable indicators of the biotic index. Our study aims to evaluate water quality and ecological status using biotic and diversity indices based on benthic macroinvertebrates. The macroinvertebrate samples used in this study were collected seasonally from 16 stations determined in Karasu Stream. These samples consist of 14747 specimens belonging to 9 orders and 40 families of Insecta, Malacostraca, Hirudinea and Oligochaeta. In addition, some physicochemical parameters were measured at the stations. The data obtained were analyzed using the Turkey-Biological Monitoring Working Group (TR-BMWP), Average Score per Taxon (ASPT), Family Biotic Index (FBI), Ephemeroptera, Plecoptera, and Trichoptera (EPT) taxon richness index, and Shannon-Wiener (SWDI), Simpson (SDI) and Hill’s diversity indices. Our results showed that the last six stations of Karasu Stream were under pressure due to various anthropogenic effects. According to biotic indexes, the ecological quality status of the water in the stations was determined to be High or Good in the first ten stations and Average, Poor, or Bad in the last six stations. The results of our study showed that TR-BMWP, FBI, and EPT indices are more reliable in determining water quality than the ASPT index and reflect the environmental situation better. Since the TR-BMWP index is adapted to macroinvertebrate taxa distributed in Türkiye, it can be used as a suitable tool for evaluating other rivers’ water quality in Türkiye.

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Using macroinvertebrate‐based biotic indices and diversity indices to assess water quality: A case study on the Karasu Stream (Kastamonu, Türkiye)

Abdulhamed M Omar Etriieki, İbrahim Küçükbasmacı

Ecohydrology, e2627, 2024

Biomonitoring is an approach that uses indicators or sentinel species to assess the health or pollution of an environment, combining diversity based on specific taxonomic groups with the taxa's indicator of pollution into a single index or score. Benthic macroinvertebrates are among the most preferred and valuable indicators of the biotic index. Our study aims to evaluate water quality and ecological status using biotic and diversity indices based on benthic macroinvertebrates. The macroinvertebrate samples used in this study were collected seasonally from 16 stations determined in Karasu Stream. These samples consist of 14,747 specimens belonging to 9 orders and 40 families of Insecta, Malacostraca, Hirudinea and Oligochaeta. In addition, some physicochemical parameters were measured at the stations. The data obtained were analysed using the Turkey‐Biological Monitoring Working Group (TR‐BMWP), Average Score per Taxon (ASPT), Family Biotic Index (FBI), Ephemeroptera, Plecoptera and Trichoptera (EPT) taxon richness index, and Shannon–Wiener, Simpson and Hill's diversity indices. Our results showed that the last six stations of Karasu Stream were under pressure due to various anthropogenic effects. According to biotic indexes, the ecological quality status of the water in the stations was determined to be high or good in the first 10 stations and average, poor or bad in the last 6 stations. The results of our study showed that TR‐BMWP, FBI and EPT indices are more reliable in determining water quality than the ASPT index and reflect the environmental situation better. Since the TR‐BMWP index is adapted to macroinvertebrate taxa distributed in Türkiye, it can be used as a suitable tool for evaluating other rivers' water quality in Türkiye.

Description

Background and aims

The physical parameters and the occurrence and abundance of zooplankton in Ain-Zayanah lagoon, Benghazi, Libya, were studied from August, 2005–July, 2006, as a part of detailed investigation on the feasibility of converting the lagoon into an aquaculture farm for captive breeding and culturing of different economically important edible fish, fish fry, molluscan and crustacean animals, and for culturing the zooplankton which form the food of these organisms.

Methods

Five different sites located near to the shore were selected in the lagoon for water and zooplankton monthly sampling and analyses. The physical parameters of the water included air and water temperatures, transparency, electrical conductivity, total dissolved solids, pH, dissolved oxygen and total hardness; they were analysed using standard methods. Various differences were revealed in the relationships of these parameters in and between sites, months and seasons.

Results

Seven major groups of zooplankton inhabited Ain-Zayanah lagoon; rotifers formed the dominant one (40.50%), followed by rhabtida (21.61%), crustacean larva (14.55%), foraminifera (13.23%), ostracoda (5.97%), copepoda (4.13%) and cladocera (0.01%). Air and water temperatures, pH, DO, electrical conductivity, TDS and hardness had different relationships with the seven zooplankton groups in Ain-Zayanah lagoon.

Conclusion

The results obtained were discussed extensively in the light of available literature in the fields, and suitable conclusions, suggestions and precautions to be taken were given in connection with the establishment of Aquaculture farm in Ain-Zayanah lagoon

Structural changes affecting cardiomyocyte function may contribute to the pathophysiological

remodeling underlying cardiac function impairment. Recent reports have shown that

endogenous nitric oxide (NO) plays an important role in this process. In order to examine the role

of NO in cardiomyocyte remodeling, male rats were acclimated to room temperature (22± 1
C) or

cold (4 ± 1
C) and treated with 2.25% L-arginine
HCl or 0.01% L-NAME (Nx-nitro-L-arginine

methyl ester)
HCl for 45 days. Untreated groups served as controls. Right heart ventricles were routinely

prepared for light microscopic examination. Stereological estimations of volume densities of

cardiomyocytes, surrounding blood vessels and connective tissue, as well as the morphometric measurements

of cardiomyocyte diameters were performed. Tissue sections were also analyzed for

structural alterations. We observed that both L-arginine and L-NAME supplementation induced

cardiomyocyte hypertrophy, regardless of ambient temperature. However, cardiomyocyte hypertrophy

was associated with fibrosis and extra collagen deposition only in the L-NAME treated group.

Taken together, our results suggest that NO has a modulatory role in right heart ventricle remodeling

by coordinating hypertrophy of cardiomyocytes and fibrous tissue preventing cardiac fibrosis.

Leishmania pyrimidine salvage is replete with opportunities for therapeutic intervention with enzyme inhibitors or antimetabolites. Their uptake into cells depends upon specific transporters; therefore it is essential to establish whether various Leishmania species possess similar pyrimidine transporters capable of drug uptake. Here, we report a comprehensive characterization of pyrimidine transport in L. major and L. mexicana. In both species, two transporters for uridine/adenosine were detected, one of which also transported uracil and the antimetabolites 5-fluoruracil (5-FU) and 5F,2'deoxyuridine (5F,2'dUrd), and was designated uridine-uracil transporter 1 (UUT1); the other transporter mediated uptake of adenosine, uridine, 5F,2'dUrd and thymidine and was designated Nucleoside Transporter 1 (NT1). To verify the reported L. donovani model of two NT1-like genes encoding uridine/adenosine transporters, and an NT2 gene encoding an inosine transporter, we cloned the corresponding L. major and L. mexicana genes, expressing each in T. brucei. Consistent with the L. donovani reports, the NT1-like genes of either species mediated the adenosine-sensitive uptake of [3H]-uridine but not of [3H]-inosine. Conversely, the NT2-like genes mediated uptake of [3H]-inosine but not [3H]-uridine. Among pyrimidine antimetabolites tested, 5-FU and 5F,2'dUrd were the most effective antileishmanials; resistance to both analogs was induced in L. major and L. mexicana. In each case it was found that the resistant cells had lost the transport capacity for the inducing drug. Metabolomics analysis found that the mechanism of action of 5-FU and 5F-2'dUrd was similar in both Leishmania species, with major changes in deoxynucleotide metabolism. We conclude that the pyrimidine salvage system is highly conserved in Leishmania species - essential information for the development of pyrimidine-based chemotherapy.

One of the most promising new targets for trypanocidal drugs to emerge in recent years is the cyclic AMP (cAMP) phosphodiesterase (PDE) activity encoded by TbrPDEB1 and TbrPDEB2. These genes were genetically confirmed as essential, and a highaffinity inhibitor, CpdA, displays potent antitrypanosomal activity. To identify effectors of the elevated cAMP levels resulting from CpdA action and, consequently, potential sites for adaptations giving resistance to PDE inhibitors, resistance to the drug was induced. Selection of mutagenized trypanosomes resulted in resistance to CpdA as well as cross-resistance to membranepermeable cAMP analogues but not to currently used trypanocidal drugs. Resistance was not due to changes in cAMP levels or in PDEB genes. A second approach, a genome-wide RNA interference (RNAi) library screen, returned four genes giving resistance to CpdA upon knockdown. Validation by independent RNAi strategies confirmed resistance to CpdA and suggested a role for the identified cAMP Response Proteins (CARPs) in cAMP action. CARP1 is unique to kinetoplastid parasites and has predicted cyclic nucleotide binding-like domains, and RNAi repression resulted in >100-fold resistance. CARP2 and CARP4 are hypothetical conserved proteins associated with the eukaryotic flagellar proteome or with flagellar function, with an orthologue of CARP4 implicated in human disease. CARP3 is a hypothetical protein, unique to Trypanosoma. CARP1 to CARP4 likely represent components of a novel cAMP signaling pathway in the parasite. As cAMP metabolism is validated as a drug target in Trypanosoma brucei, cAMP effectors highly divergent from the mammalian host, such as CARP1, lend themselves to further pharmacological development